Pulse Industry

Project Abstract: Developing dry bean cultivars and germplasm with high yield, disease resistance and marketable seed quality

The objectives of this activity are to coordinate and conduct the annual Long Season Wide Row Dry Bean Cooperative Registration Trials and the Regional Variety Trials, improve dry bean disease resistance with a focus on common bacterial blight, anthracnose and white mould in major bean market classes, develop molecular markers for seed hardness, evaluate amino acid compositions and agronomic performance of a genetic population derived from SMARC1NPN1/Morden003, and continue breeding selection for improved yield, seed quality and early-maturity for production in Manitoba.

Advanced breeding lines from public and private breeders were successfully tested annually in the Long Season Wide Row Dry Bean Cooperative Registration Trials at four locations in Manitoba. The trials were conducted with 5 m four-row plots with three replications in a randomized complete block design. The entries were also evaluated for resistance to common bacterial blight, anthracnose and white mould.

The results have been submitted annually to the Prairie Recommending Committee for Pulses and Special Crops of the Prairie Grain Development Committee to support variety registration in Canada. In collaboration with the Manitoba Pulse & Soybean Growers, registered dry bean varieties or advanced breeding lines were tested at Morden as part of the Regional Trials. The results have been published in Seed Manitoba and Pulse Beat to provide production guides to regional pulse growers and the pulse industry. Breeding selections were made in diverse market classes and elite selections have been tested in the advanced and Registration trials. Three lines have been supported for registration. Working with Canterra Seeds Inc., pedigreed seed of CR318-6 is in production in Idaho and Washington State for commercialization. Stone seed is a dry bean production concern in Manitoba. A recombinant inbred line population was developed from a hard and a soft parental line and was evaluated at two locations in Manitoba in 2015 and 2016. Seeds were hand-harvested and subjected to hydration analysis. Genomic DNA sequencing and SNP calls were conducted at the Cornell University genomic sequencing facility. A total of 92,800 SNPs were generated from the sequencing project, and 5,038 SNPs were selected and used for the quantitative trait locus (QTL) mapping. As results, three QTLs have been mapped on chromosomes Pv1, Pv2 and Pv7. The major QTL on Pv7 explains approximately 45% of the stone seed phenotypic variation. The two minor QTLs on Pv1 and Pv2 each explained over 14 % of the variation, which is also significant. We are in the process of fine-mapping of the QTLs, in order to identify the candidate genes and develop molecular markers associated with seed hardness. To investigate the feasibility of improving dry bean protein quality, 180 lines of an F2:8 recombinant inbred line population derived from SMARC1NPN1/Morden003 were grown with three replications at two locations (Morden, MB and London, ON). Amino acid analysis was conducted for selected lines grown at the two locations. Some lines resembling the protein profiles of SMARC1N-PN1 were characterized by a substantial increase in cysteine concentration as compared with Morden003. The top line, W09-01-01-84, had a 40% increase in cysteine, versus Morden003, averaged over two years and two locations. Anthracnose resistance screening identified lines with resistance to races 73 and/or 105. Preliminary mapping with SNPs confirmed the resistance in Morden003 at the Co-3 locus and SMARC1N-PN1 at the Co-1 locus. Genetic analysis and mapping of various agronomic traits are in progress by a master degree graduate student at Western University. Unique materials have been used in crossing for future variety/germplasm development. The research results from this project have been reported in four scientific manuscripts and ten conference presentations.

Project lead: Dr. Anfu Hou (204) 822-7228 anfu.hou@agr.gc.ca


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