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Project Abstract: Improving Nutritional Values of Dry Bean to Promote Utilization in Health Foods

Dry bean has been identified as a health food for decades and its nutritional values are highly important in human health. In addition to protein, dry bean contains significantly high amounts of resistant starch and dietary fiber, which offers new market opportunities for novel end-uses of dry bean components for functional food industry. Dry bean produced in Canada, however, is primarily exported as a raw commodity and its nutritional values have not been fully explored and utilized. Therefore, the identification and utilization of dry bean with high resistant starch and dietary fiber will greatly improve its nutritional values in health food applications.

The objective of this sub-activity is to characterize the contents and variation of resistant starch and dietary fibers in dry bean germplasm, cultivars and advanced lines and further identify dry bean genotypes with high resistant starch and dietary fiber to improve the nutritional values by pyramiding resistant starch and dietary fiber.

For methodology, dry bean samples from diverse market classes were collected from dry bean breeding programs across Canada and the USDA gene bank. Resistant Starch Assay Kit was used to determine resistant starch content and Total Dietary Fibre Assay Kit was used to determine dietary fiber content. The dry bean field trials were conducted in 2014, 2015 and 2016 in AAFC-Lethbridge. Over 350 dry bean samples from diverse market classes including germplasm, cultivars and advanced breeding lines were collected and analyzed for protein, total starch, resistant starch and dietary fiber in 2015 and 2016.

Based on the overall analytic results, 2 germplasm lines with high protein and high dietary fiber contents, 1 great northern bean cultivar with high dietary fiber content and 2 germplasm lines with high resistant starch content were selected and 16 cross combinations were made between these 5 genotypes in September 2016. The F1 hybrid seeds were harvested in December 2016, and will be advanced to F4 generations in 2017, and F5 populations of recombinant inbred lines (RILs) by the end of the growing forward-2 term.

The progress of this sub-activity will support AAFC dry bean breeding program to develop novel dry bean cultivars with high protein, high resistant starch and high dietary fiber for functional food applications.


Project lead: Dr. John Lu (403) 317-3302 zhen-xiang.lu@agr.gc.ca

 

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